River Water Part 1

NGSS Phenomena: An animation of a stream, with the water gently rippling.
On a hike with your friend Lianna, she runs out of water and tells you to hold on a second while they grab some water from the river next to the trail. You run after them, telling them that it’s not safe because there are a lot of dangerous things living in the water! Your friend doesn’t believe you, but agrees not to drink the water and you both decide to take a water sample back to class to test it.






At school, you have a microscope that allows you to see objects at 10x, 40x, and 400x. Your teacher tells you that at the maximum magnification, you’ll able to see the shape of objects that are 2-5 µm, but the depth of field is very small at that magnification.






120 nm Large virus (includes influenza virus)




150-250 nm small bacteria such as Mycoplasma




200 nm (200 to 500 nm) Lysosomes




(1 - 10 µm) the general sizes for Prokaryotes




2 µm E.coli - a bacterium




5 µm length of chloroplast




6 µm (3 - 10 micrometers) the Nucleus




9 µm Human red blood cell




(10 - 30 µm) Most Eukaryotic animal cells




(10 - 100 µm) Most Eukaryotic plant cells




90 µm small Amoeba




120 µm Human Egg




up to 800 µm large Amoeba


120 mm Diameter of an ostrich egg








Key:



1 millimeter (mm) = 1000 micrometers (µm)



1 micrometer (µm) = 1000 nanometers (nm)

1. Once you have the water you want to test, you place it onto a slide and fit it into the microscope. You’ll first want to use the magnification, which is in order to on what is on the slide.

Drag the answers below into the grey placeholders above

lowest
light up
400x
focus
40x
highest
100x
2x

2. Lianna, who didn’t believe you that there was anything in the water, looks into the microscope at 100x and sees absolutely nothing. After moving the slide around on the microscope, they still see no shapes in the water and then look at 400x to see nothing but white light. They conclude that the water must not have any living things.



Explain at least 2 issues that may have prevented their investigation from being successful and how you could improve the investigation in the future.

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